Re-analysing database for novel targets
Keywords
siRNA
Statistics & modeling
Challenge
A customer was interested in finding new potential targets for siRNAs based on their existing databases.
Solution
BISC Global performed a study combining following analyses:
- siRNA hit calling and determining on/off targets
- Selection of siRNA’s for subsequent toxicity screen
- Define thresholds for cell viability, toxicity, household gene stability
Impact
BISC Global identified compelling on/off-target interactions with limited effects on cell viability and toxicity.