Single nucleus sequencing of multinucleated muscle fibers reveals unique insights into normal and disease-associated regeneration

KEYWORDS: scRNA-Seq & bulk RNA-seq | Therapeutic targets discovery | Public datasets | Muscle disorder

Challenge

Muscle weakness and wasting debilitate many individuals globally. Our customer was looking for therapeutic targets to prevent & reverse a specific muscle degeneration process.

Solution

Analysis of a large-scale single nucleus RNA-seq dataset (~350K cells) revealed (rare) cell (sub)types and enriched/depleted genes in association with the muscle disease.

Impact

Thanks to the results of our analysis, the customer can focus on the myogenic and connective cell types, as well as the enriched/depleted genes in the search for a therapeutic candidate against the muscle disease.